Manhattan, Kansas, USA
March 20, 2015
Kansas State University researchers are releasing a study that details the first haplotype map of wheat. The map is a genetic breakthrough for improving wheat varieties around the world. The study was led by Eduard Akhunov, associate professor of plant pathology, center, and Katherine Jordan, left, and Shichen Wang, research associates and lead authors.
Kansas State University scientists have released findings of a complex, two-year study of the genomic diversity of wheat that creates an important foundation for future improvements in wheat around the world.
Their work has produced the first haplotype map of wheat that provides detailed description of genetic differences in a worldwide sample of wheat lines. In genetics, a haplotype map is a powerful tool for transferring sequence-level variation to multiple gene mapping projects.
"All of these new, genomic-based strategies of breeding promise to significantly accelerate breeding cycles and shorten release time of future wheat varieties," said Eduard Akhunov, associate professor of plant pathology and the project's leader.
Plant scientists often look at the genetic makeup of an organism to breed new varieties for specific, desirable traits, such as drought, pest or disease resistance. Akhunov said the haplotype map gives scientists quick access to rich, genetic variation data that increases the precision of mapping genes in the wheat genome, and improves scientists' ability to select the best lines in breeding trials.
Akhunov's research associates, Katherine Jordan and Shichen Wang, are lead authors of the study, "A haplotype map of allohexaploid wheat reveals distinct patterns of selection on homoeologous genomes," which will be published in an upcoming issue of the journal Genome Biology. The project was coordinated through the International Wheat Genome Sequencing Consortium, and included groups in Canada, Australia, the U.K. and the U.S. Much of the work took place in Kansas State University's Integrated Genomics Facility.
The study included 62 wheat lines from around the world that were either modern cultivars or varieties not previously improved through formal breeding techniques, called landraces.
To reduce the complexity of the wheat genome, the research team developed a tool called "exome capture assay" to perform targeted sequencing of only functional parts of the larger wheat genome. This technique bypasses those parts of the genome that are repetitive, according to Akhunov.
The scientists found 1.6 million locations — called single nucleotide polymorphisms — in the genome where the wheat lines differed from one other. The research team used this information to describe the impact of these differences on the function of tens of thousands of wheat genes.
"Once genes controlling agronomic traits are identified, they can be used for improving wheat using not only traditional breeding approaches, but also new strategies that are based on biotechnology and molecular biology," Akhunov said.
"In the future, we will expand the set of wheat lines characterized using our sequencing strategy by including not only more genetically and geographically diverse wheat lines, but also by including close and distant relatives of wheat," he said. "These wheat relatives are known for being a reservoir of valuable genes for agriculture that can improve abiotic and biotic stress tolerance or other quality traits, and increase yield."
Akhunov said that genomics-based approaches are now being introduced into every wheat breeding program worldwide. "I am sure that we will see the impact of diversity resources developed at Kansas State University on wheat breeding within three to five years," he said.
In addition to Jordan and Wang, the Kansas State University research team included Alina Akhunova and Yanni Lun, who coordinated work in the Integrated Genomics Facility; and plant pathology faculty member Christopher Toomajian.
"This study would be impossible without the computational and genomic infrastructure developed by K-State Research and Extension, the College of Agriculture and Kansas State University over the last five to seven years," Akhunov said.
Funding for the study was provided by the U.S. Department of Agriculture's National Institute of Food and Agriculture through the National Research Initiative.
Investigadores crean el primer mapa genético de variedades de trigo del mundo
Source: Fundacion Antama
May 18, 2015
Científicos de la Universidad Estatal de Kansas han lanzado el primer mapa genético de variedades trigo con información detallada de las diferencias genéticas de una muestra mundial de diferentes líneas de trigo. El estudio incluye un total de 62 líneas de trigo de todo el mundo en las que se encuentran variedades modernas así como variedades locales que no han sido mejoradas a través de técnicas de reproducción.
Para reducir la complejidad del genoma del trigo, el equipo de investigación ha desarrollado una herramienta llamada “Ensayo de captura exoma” para llevar a cabo la secuenciación selectiva de sólo algunas partes funcionales del genoma del trigo. Esta técnica permite evitar las partes repetidas del genoma. El equipo encontró 1,6 millones de ubicaciones (polimorfismos de un solo nucleótido) en el genoma donde las líneas de trigo diferían entre sí. Utilizaron esta información para describir el impacto de estas diferencias en las funciones de decenas de miles de genes de trigo.
“En el futuro, vamos a ampliar el conjunto de líneas de trigo caracterizadas utilizando nuestra técnica de secuenciación incluyendo no sólo más líneas genética y geográficas de trigo, sino también incluyendo parientes cercanos y lejanos de trigo. Estos parientes son conocidos por ser una reserva de genes muy valiosa para la agricultura, que puede mejorar la tolerancia al estrés abiótico y biótico u otras características de calidad, así como aumentar el rendimiento “, explica Eduard Akhunov, profesor asociado de patología vegetal y líder del proyecto.
“Una vez que se identifican los genes que controlan los rasgos agronómicos, éstos pueden ser utilizados para mejorar el trigo utilizando no sólo los enfoques tradicionales de cultivo, sino también nuevas técnicas basadas en la biotecnología o la biología molecular”, resalta Akhunov.
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