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An updated version of the International Seed Health Initiative (ISHI) protocol “Detection of Paracidovorax citrulli in Cucurbit Seeds by SE‑qPCR” is now available on the ISF website
January 2026
Source: International Seed Federation (ISF) News
An updated version of the International Seed Health Initiative (ISHI) protocol “Detection of Paracidovorax citrulli in Cucurbit Seeds by SE‑qPCR” is now available on the ISF website.
Paracidovorax citrulli is a seed-transmitted bacterial pathogen affecting several Cucurbitaceae species, including watermelon, melon, cucumber, squash, and pumpkin. It is the causal agent of bacterial fruit blotch, a disease with outbreaks reported worldwide. Reliable seed health testing using robust detection methods remains essential for effective disease management.
The revised protocol introduces several important updates:
- Removal of the ZUP qPCR assay with detailed justification provided in a newly published addendum validation report.
- Updated pathogen nomenclature (formerly Acidovorax citrulli) in line with current classification.
- Revised control names to align with the latest ISHI Best Practices.
- Correction of one primer sequence.
These revisions help ensure that the protocol remains current, scientifically sound, and fully aligned with the most up-to-date standards in seed health testing.
More news from: ISF - International Seed Federation*
Website: http://www.worldseed.org Published: January 30, 2026 |
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